Donkey Kong Island is the home of Donkey Kong and his friends. It is also where Donkey Kong Country takes place. Over the years, this location has undergone more than one major refurbishment, as is clearly evident in future installments of the Donkey Kong Country franchise such as Donkey Kong 64 and Donkey Kong Country Returns.
 Donkey Kong Country
- Kongo Jungle
- Monkey Mines
- Vine Valley
- Gorilla Glacier
- Kremkroc Industries, Inc.
- Chimp Caverns
- Gangplank Galleon
Bosses are found at the end of each area. There is one for each area. Gangplank Galleon only has one level, so that is naturally where the boss fight occurs.
- Very Gnawty - Kongo Jungle
- Master Necky - Monkey Mines
- Queen B. - Vine Valley
- Really Gnawty - Gorilla Glacier
- Dumb Drum - Kremkroc Industries, Inc.
- Master Necky Sr. - Chimp Caverns
- King K. Rool - Gangplank Galleon